Download Citation on ResearchGate | Actinobacillus seminis isolated from cattle | The organism commonly referred to as Actinobacillus. Actinobacillus seminis is a well known cause of epididymitis in Australian rams ( Baynes and Simmons, ) and has also been reported as associated with. The main causative agents of ovine infectious epididymitis are Brucella ovis, Actinobacillus seminis, and Histophilus somni [1–3].
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Subcellular fractions of A. The proteins from the 2 isolates had a similar molecular mass. Immunoblot analysis of Actinobacillus seminis microvesicles, showing recognition of the kDa protein by antibody against A.
Actinobacillus seminis is a pleomorphic, gram-negative bacillus that is a well-known cause of epididymitis in Australian rams Baynes and Simmons,and has been documented in the United States of America, South Africa, New Zealand, Hungary, Patagonia, the United Kingdom, Turkey, Kidney, and Spain.
Interestingly, there was a low frequency of bacterial isolation at the inoculation site, which suggests that the chronicity of infection in the tail of the epididymis may prevent bacterial isolation [ 24 ].
The most severe microscopic lesions were observed at the site of inoculation i. Subclinical cases have been known atcinobacillus persist for up to 4 years. This is the first comparative study between A. Al-Katib and Actiobacillus, A, extracts of each bacterium were exposed to antibody against A. Furthermore, when semiis type of hyperimmune serum of actinobacilllus inoculated with whole cells of B.
Membrane microvesicles of A. All rams were confirmed as free of infectious epididymitis by B. The values of each point represent the mean. While some Australian flocks may have attempted actinnobacillus eradicate A. Group 2 had a unique protein profile, with relatively few protein bands in common. Rams that did not show libido at some point of the trial period were subjected to electroejaculation [ 13 ].
This article has been cited by other articles in PMC. The results suggest that the kDa protein could be used to establish a diagnostic test specific actinkbacillus ovine epididymitis caused by A. Twenty rams of age 18 to 24 months were infected by intraepididymal inoculation of A. In the Australian context, epididymitis is the most important manifestation of A.
However, even without differentiating the etiological agent, clinical examination is essential as a screening procedure, because it allowed the detection of signs of infectious epididymitis. Infectious epididymitis in rams is a major disease in countries devoted to the industrialized breeding of sheep. Am J Vet Res. Changes frequently observed include abscess in the left epididymis tail, which was the site of inoculation Figures 5 a and 5 band an increase in size of inguinal and iliac lymph nodes Table 2.
Severe testicular degeneration may impact directly on fertility of the animal. The Scientific World Journal.
Furthermore, these two organisms actknobacillus indistinguishable clinical, gross, or microscopic findings. Thickening and fibrous adherence of tunica albuginea with tunica vaginalis were also frequent changes Figure 6 d. The objective of our study was to identify a protein specific to A. After 7 d, the rabbits were given intramuscular booster inoculations without adjuvant 3 times, at 1-wk intervals.
Can J Vet Res. Nonparametric data testicular consistency and length and width of the tail of epididymes were analyzed by Kruskal Wallis, followed by Dunn test for multiple comparisons. Additionally, in the case of H. This study aimed to investigate clinical and pathological changes associated with experimental infections with A. Identification and characterization of microorganisms has historically been based on morphologic, biochemical, serologic, toxigenic, and genetic characteristics.
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B, Western-blot results, showing recognition of the kDa protein in A. Results Prior to inoculation, all 20 rams were clinically healthy, without palpable lesions in the testes or epididymides. Actinobacillus seminis is a well known cause of epididymitis in Australian rams Baynes and Simmons, and has also been reported acginobacillus associated with metritis, polyarthritis Higgins et alposthitis and mastitis Watt et al Both groups seminks two months of adaptation and training for semen collection using an artificial vagina.
All antibodies were produced in rabbits with the use of sonicated cells.
Immunohistochemical technique used was only effective for immunolabeling A. This disease is a serious reproductive disorder, with marked pathological changes in the genital tract 2.
View at Google Scholar I. The organism has been recovered from placenta and uterus of ewes after natural and experimental infections Al-Katib and Dennis, To confirm the infection, semen, urine, and blood were obtained prior to inoculation and every seven dpi, during six weeks.